Figure 6
Effect of SCN− supplementation on the extent of formation of HOCl-modified proteins. The graphs show the differences in: (A) mean fluorescence intensity from 2D10G9 antibody epitopes, and (B) the percentage of fluorescence with an intensity over 350 compared to the background fluorescence (over 150) of the individual section, as a representation of the extent of 2D10G9 epitope recognition for HOCl-modified proteins, as measured on sections taken from the ligation site to the apex for control (black line) and SCN− (grey line) groups. Data represent mean ± SEM of n ≥ 6 rats with * showing a significant difference between vehicle/IR and SCN– supplemented/IR groups using 2-way ANOVA with a Sidak post-hoc test. Panels C and D show representative fluorescent images of the 2D10G9 epitopes taken 1800 µm from the site of ligation from: (C) a vehicle/IR rat, and (D) a SCN– supplemented/IR rat, with the images recorded and displayed with the same instrument settings.
