Figure 5

Palmitic acid induced TLR4 recruitment to membrane lipid rafts. Human SH-SY5Y cells were treated with palmitic acid, DHA or placebo during 4 h and fixed for immunohistochemistry analysis by confocal microscopy using antibodies directed towards CTXB (marker of membrane lipid rafts) or TLR4. The arrow in the merge indicated the co-localization of TLR4 and CTXB. Images were captured using a confocal microscope with high-magnification scan (X40). The figure is representative of two biological replicates and each image resulted from several slides that were analyzed by confocal microscopy.