Figure 2 | Scientific Reports

Figure 2

From: Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluation

Figure 2

Characterization of adipocytes in monoculture. (a) Cytometer live/dead, FABP4, and CD200 staining of adipocytes after 14d differentiation. Oil Red O staining showing lipid storage in adipocytes after differentiation and 14-day treatment in (b) Healthy BMM (c) Diabetic BMM (d) Obese BMM (e) Proinflammatory BMM (f) Diabetic + proinflammatory BMM (g) Obese + proinflammatory BMM). (h) Quantification of lipid droplet size using ImageJ particle analysis. ICC quantification of insulin resistance biomarkers after 14d in (i) Healthy BMM (j) Diabetic BMM (k) Diabetic + Obese BMM (l) Diabetic, obese, and proinflammatory BMM. Statistical analysis performed as multi-way ANOVA followed by Dunnett’s test (α = 0.05) using the coverslip-wide average of two independent coverslips per condition.

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