Figure 6
Oil Red O staining showing lipid accumulation in hepatocytes after 14 days in recirculating diabetic, obese, proinflammatory BMM with (a) Control (b) 30 μM Metformin. (c) Quantification of effect of metformin for each medium condition. Oil Red O staining showing lipid accumulation in hepatocytes after 7 days in recirculating diabetic, obese, proinflammatory BMM with (d) Control (e) 1 mM Metformin. (f) Quantification of the effect of metformin for each medium condition. Statistical analysis was performed with a one-way ANOVA followed by Dunnet’s test with the relative change in each condition to block for TNF-α addition and lipid addition (α = 0.05) using between 2 and 4 independent coverslips (e) or 3 independent coverslips per condition (f).
