Figure 2
Transduction efficiency of bone marrow cells with LV-BOS-YFP in SOD1(G93A) mice. (a,b) Representative flow cytometric analysis of LV-BOS-YFP-mediated transduction in SOD1(G93A) mice at 3 weeks [(a), 11-week-old] and 6 weeks [(b), 14-week-old] after bone marrow transplantation. Red letters indicate the YFP-positive population in (a) and (b). (c) The percentage of YFP positive WBCs in total peripheral WBCs of 3 weeks and 6 weeks after bone marrow transplantation (n = 5, each group). (d) YFP signals (green) and Nissl stain (red) in ventral horn sections of the spinal cord at the lumbar level from SOD1(G93A) mice at 6 weeks after bone marrow transplantation with LV-BOS-YFP gene transduction. The left panel shows LV-BOS empty vector group and the right panel shows the LV-BOS-YFP vector group. Scale bar = 100 μm. Error bars represent the mean + SD. *p < 0.05. WBCs white blood cells.
