Figure 4
Accumulation of microglia expressing YFP and quantitative analysis of YFP signals in the spinal cord of SOD1(G93A) mice after transplantation. (a) YFP (green) and Iba1 (red) signals in ventral horn sections of the spinal cord at the lumbar level in SOD1(G93A) mice at 0 weeks (left panels, 8-week-old), 6 weeks (middle panels, 14-week-old) and 10 weeks (right panels, 18-week-old) after bone marrow transplantation with gene transduction by LV-CD68-YFP. Upper panels show low magnification images of spinal cords and lower panels show a high magnification image of the square in the upper panel of the same column. Scale bar = 50 µm. (b) Number of Iba1 and YFP positive cells (Iba1 + YFP +) and Iba1 positive and YFP negative cells (Iba1 + YFP −) in microglia of the spinal cord of SOD1(G93A) mice at 0, 6, and 10 weeks after bone marrow transplantation with gene transduction by LV-CD68-YFP (n = 5, each group). (c) Population of YFP positive cells in Iba1 positive cells in microglia of the spinal cord of SOD1(G93A) mice at 0, 6, and 10 weeks after bone marrow transplantation with gene transduction by LV-CD68-YFP (n = 5, each group). Error bars represent the mean + SD. **p < 0.01.
