Figure 7
Histological analysis of motor neuron survival and astrogliosis in spinal cord, degeneration of muscle and peripheral nerve in SOD1(G93A) mice after treatments. The first and second row from the top: Nissl stain (red) with YFP (green) signals of the spinal cords in the SOD1(G93A), SOD1(G93A) + LV-CD68-YFP and SOD1(G93A) + LV-CD68-GLT1-YFP mice at 18–20-week-old. Upper row shows the color images and lower row shows black and white images of red color (Nissl staining) isolated from the corresponding upper row. The third and fourth row from the top: GFAP immunohistochemistry (red) with YFP (green) signals of the spinal cords in the same three group. Upper row shows the color images and lower row shows black and white images of red color (GFAP staining) isolated from the corresponding upper row. The third and fourth row from the bottom: upper row shows YFP (green) signals with a nuclear stain (DAPI, blue) of the spinal cords in the same three group. Lower row shows Hematoxylin–eosin stain of anterior tibial muscle in the same three group. The first and second row from the bottom: S100 immunohistochemistry (red) with YFP (green) signals of the spinal cords in the same three group. Upper row shows the color images and lower row shows black and white images of red color (S100 staining) isolated from the corresponding upper row. Scale bar = 100 µm. The bar graph shows relative intensity of Nissl, GFAP and S100 staining as seen in the same three group (n = 5 in each group). The intensity of Nissl, GFAP and S100 staining was measured in the black and white image using the Image J software and the ratio was calculated against the intensity of that in SOD1(G93A) group. The bar graph shows relative area of muscle fiber in the same three groups. The average areas of muscle fibers were compared among the three groups (n = 5 in each group). Error bars represent the mean + SD. **p < 0.01 between the treatment group and others.
