Figure 2

Exhaustion T cells were abundantly detected in HER2-low BC-M. Frequency of PD-1⁺CD25⁻ subset in CD4⁺ or CD8⁺ gated T cells in the spleen (A) and bone marrow (B) of NOG-hIL-4-Tg mice transplanted with HD (n = 22) or BC (HER2 0; n = 3, HER2 1 + &2 + ; n = 8, HER2 3 + ; n = 1) PBMCs. HD and BC-transplanted mice are indicated as white (HD) or black bars (BC). *p = 0.05, **p = 0.01. BC breast cancer; HD healthy donor; HER2 Human epidermal growth factor receptor 2; PBMCs peripheral blood mononuclear cells.