Figure 3
Comparison of human proteins in mouse plasma. (A) Principal component analysis (PCA) of human proteins in plasma of mice transplanted with HD (green, PBS; n = 4, CH401MAP; n = 4) or BC (red, PBS; n = 6, CH401MAP; n = 6). t-Predicted score scatterplot shows the results. The scaffold software used for PCA was SIMCA version 3 (https://www.sartorius.com/en/products/process-analytical-technology/data-analytics-software/mvda-software/simca?gclid=EAIaIQobChMI_7fm_JHY8AIVxcKWCh3A6AnCEAAYASAAEgKbEvD_BwE). Sample names are shown in Table 1. R2X[2] = 0.152; R2X[4] = 0.0974. The components are shown in Table S2. Relative amount of each human antibody class in (B) HD (open circle; n = 4) or BC (filled circle; n = 6) plasma and (C) HD-transplanted (open circle; PBS; n = 4, CH401MAP; n = 4) or BC-transplanted (filled circle; PBS; n = 6, CH401MAP; n = 6) mice. HER2 3 + BC samples in BC-Ms were labeled with dotted squares. *p = 0.05. BC breast cancer; HD healthy donor; PBMCs peripheral blood mononuclear cells; PBS phosphate-buffered saline; Ig immunoglobulin.
