Figure 4
Production of specific antibody was decreased in BC immune environment. Concentrations of anti-CH401MAP antibody in plasma of HD-M and BC-M. (A) Transition graph of anti-CH401MAP antibody in mice transplanted with PBMCs of HD (n = 17) or BC (n = 12). The concentration was compared between PBS (filled circle) or CH401MAP (open circle) groups, and the lines represent the mice transplanted with the same donor PBMC. (B) Anti-CH401MAP antibody concentration in supernatant and percentage of anti-CH401MAP antibody detected in wells in a culture plate. HD- or BC-transplanted spleens were fused with P3X (n = 5). As the hybridoma yield is different between each experiment, positive cell ratio was calculated based on the sum of all experiments. BC breast cancer; HD healthy donor; PBMCs peripheral blood mononuclear cells; PBS phosphate-buffered saline.
