Figure 3

Blocking nTRIP6 function does not affect myoblast proliferation and cell cycle exit. C2C12 cells transfected with either the control (cBP) or the blocking peptide (BP) were subjected to a differentiation experiment. (a) Cells were counted at the indicated day and results are shown relative to the cBP-transfected cells at day-2 and are mean ± SD of three independent experiments. Individual values are depicted by symbols, each representing an independent experiment. (b, c) Cells were pulsed with EdU for 1 h at the indicated time point, fixed, stained for EdU incorporation and peptide expression (mCherry) and analysed by confocal microscopy. The percentage of EdU-positive nuclei among transfected cells (mCherry positive) is presented as mean ± SD of three independent experiments (b). Individual values are depicted by symbols, each representing an independent experiment. Representative images are shown (c, scale bar: 200 µm).