Figure 4

Blocking nTRIP6 function delays late differentiation and fusion. Control C2C12 cells transfected with either the mCherry-tagged, nuclear targeted nTRIP6 blocking peptide (BP) or the control, scrambled version of the peptide (cBP) were subjected to a differentiation experiment. Cells were fixed at the indicated day and subjected to immunofluorescence analysis using antibodies against MYH3 and mCherry. Nuclei were counterstained using DAPI. (a) Representative images are presented (scale bar 100 µm). (b) The percentage of MYH3 expressing cells among transfected cells (mCherry-positive), (c) the fusion index (percentage of mCherry positive nuclei within fused myotubes), and (d) the number of mCherry-positive nuclei per myofiber are presented as mean ± SD of three independent experiments.