Figure 6

Skeletal muscle regeneration in Trip6 knockout mice. (a) Schematic representation of the protocol. The Trip6 gene was knocked out in satellite cells by intraperitoneal injection of tamoxifen in Trip6^fl/fl; Pax7^Cre-ERT2/wt mice and muscle degeneration was induced by injection of notexin in the M. soleus. (b-g) The regenerating and uninjured contralateral muscles of Trip6^scko (ko) and Trip6^fl/fl control animals (fl/fl) were harvested at the indicated day post-injury (dpi). Muscle sections were stained with anti-Laminin, anti-MYOD and anti-Ki67 antibodies and counterstained with DAPI. (b) Representative images at 14 dpi are shown (the full set is presented in Supplementary Fig. S6; scale bar: 30 µm). (c) Number of MYOD-positive mononuclear cells normalized to the number of myofibres; (d) Number of MYOD-positive mononuclear cells expressing Ki67 normalized to the number of myofibres; (e) Number of MYOD-positive mononuclear cells not expressing Ki67 normalized to the number of myofibres; (f) distribution of the number of centrally located nuclei detected on myofibres cross-sections; (g) Minimum Feret’s diameter of the regenerating myofibres expressed as percent of the contralateral uninjured muscle. Bonferroni corrected P values are presented (5 animals per group).