Figure 3
(a) Schematic of generation of truncated tryptic peptide V263TCVVVDVSHEDPEVK from substituted peptide TPK/R262VTCVVVDVSHEDPEVK in mAb X. (b) Extracted ion chromatogram of native peptide TPE262VTCVVVDVSHEDPEVK and truncated peptide V263TCVVVDVSHEDPEVK in FBV and MV of mAb X. Signals from both the peptides are visible in FBV, while only the native peptide is present in MV. (c) PCR amplification plot showing the amplification of primers containing the wild-type sequence and with the sequence containing the SNP corresponding to the E262K SV.
