Figure 4

(a) Overlay of CEX profiles of mAb X and purified E262K containing mAb X (mAb X’). (b) EIC of the truncated peptide (E262K peptide) V₂₆₃TCVVVDVSHEDPEVK₂₇₈ and native peptide TPE₂₆₂VTCVVVDVSHEDPEVK₂₇₈ in mAb X’ (upper panel) and mAb X (bottom panel). EIC signal corresponding to truncated peptide and native peptide is marked in mAb X’ chromatogram while only the EIC signal corresponding to native peptide was detected and marked in mAb X chromatogram. (c) Schematic showing the generation of native and truncated peptide by tryptic digestion of mAb X’.