Table 3 Recovery of EK and Native peptide in triple-Q MS using SRM.
Amount (p moles) | Peak area | Calculated amount from linear plot (pmoles) | %Recovery (actual/calculated) |
|---|---|---|---|
EK peptide | |||
0.065 | 68,305 | 0.062 | 1.0 |
0.130 | 186,779 | 0.146 | 0.9 |
0.325 | 390,302 | 0.292 | 1.1 |
0.651 | 947,436 | 0.690 | 0.9 |
3.255 | 4,829,049 | 3.462 | 0.9 |
6.510 | 9,465,545 | 6.773 | 1.0 |
32.550 | 43,326,864 | 30.954 | 1.1 |
65.100 | 83,221,433 | 59.444 | 1.1 |
Native peptide | |||
126.187 | 86,146,234 | 118.632 | 1.1 |
252.375 | 182,163,787 | 277.256 | 0.9 |
378.562 | 257,317,432 | 401.412 | 0.9 |
504.749 | 327,136,398 | 516.756 | 1.0 |
630.936 | 407,050,857 | 648.777 | 1.0 |
757.124 | 459,068,585 | 734.712 | 1.0 |
883.311 | 518,580,860 | 833.029 | 1.1 |
1009.498 | 585,917,249 | 944.271 | 1.1 |
