Figure 5
In vivo efficacy test. (A) Scheme of the animal challenge experiment using IFN-α/β/γR KO C57BL/6 mice. (B) Survival of infected mice after viral challenge. A group of five mice were infected with 2.0 × 106 FFU of DENV-3. Statistical significance was analyzed using a log rank test. *P < 0.01 for the comparison between isotype control and 3G9-original. **P < 0.05 for the comparison between 3G9-original and the Fc-modified 3G9. There was no significant difference among the Fc-modified 3G9 (P > 0.05). (C) Scheme of the animal challenge experiment carried out using immunocompetent BALB/c mice. (D) Viremia levels of the infected mice after the viral challenge. A group of five mice were infected with 1.0 × 106 FFU of DENV-2. The limit of detection (L.O.D) was 100 FFU/ml. Viremia levels below the detection limit are plotted as half of the detection limit (50 FFU/ml). Statistical significance was analyzed using a one-way ANOVA. P < 0.05 was considered significant. n.s. not significant.
