Figure 6 | Scientific Reports

Figure 6

From: An affinity-matured human monoclonal antibody targeting fusion loop epitope of dengue virus with in vivo therapeutic potency

Figure 6

Competition ELISA and ADE assay. (A) Competition ELISA. Mouse monoclonal antibodies 4G2, 7F4, or 15C12 at 1 μg/ml were mixed with serially diluted 3G9 and incubated in a DENV-coated ELISA plate. The OD relative to that of the no competition well (without 3G9) is shown. Each data represents the average of two independent experiments. (B) Competition ADE. 100 ng/ml of 4G2 and 1:640 diluted mouse serum, which showed the peak level of enhancement, were mixed with DENV-2 NGC and serially diluted 3G9-N297A. The number of infected cells relative to that of the no competition wells is shown. Each data represents the average of two independent experiments.

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