Figure 1
From: Identification of cis-acting determinants mediating the unconventional secretion of tau
Tau hyperphosphorylation induces toxicity to Drosophila melanogaster upon expression in the retina. (a) Schematic illustration of the experimental setup and the parameters used to assess the toxicity in Drosophila retinal cells. (b) Representative images from Drosophila melanogaster eye phenotypes upon expression of different constructs under the GMR-Gal4 promoter element. (c) The severity of roughness in the eye phenotype was quantified using the Flynotyper ImageJ-based plugin and then normalized to the GFP control. Bars represent mean values ± s.d., n ≥ 6 animals/genotype were assessed. (d) Sarkosyl-soluble and insoluble fractions were isolated from transgenic Drosophila melanogaster brain homogenates and blotted against pan-tau and GFP, while actin was used as loading and assay quality control.
