Figure 1 | Scientific Reports

Figure 1

From: Gelsolin and dCryAB act downstream of muscle identity genes and contribute to preventing muscle splitting and branching in Drosophila

Figure 1

Translational profiling of embryonic muscles using TRAP. (A) Schematic representation of cell-specific polysome capture during TRAP. (B) TRAP-targeted muscle populations revealed by anti-GFP immunostaining of stage 16 Slou > RpL10a-eGFP and Lms > RpL10a-eGFP embryos. (C) Pie chart showing distribution of GO terms (biological processes) for genes commonly up-regulated (orange) or down-regulated (blue) in Slou- and-Lms positive muscles and in all time windows. (D) Bar plots showing number of genes whose expression is commonly up (FC > 2) in individual muscle subsets and in all muscles (dark red and dark blue) versus genes up-regulated exclusively in Slou (light red) and Lms (light blue) populations. Proportion of genes specific for each population at each time point are shown in light color boxes. (E) Heatmap showing the transition profiles of gene expression in Lms and Slou populations. Heatmap was generated using R package Pheatmap version 1.0.12 ; URL: https://cran.r-project.org/web/packages/pheatmap/index.html. Tr1 represents gene expression transition from the time window T1 to T2 and Tr2 the transition from T2 to T3. Cluster 1 (green) and cluster 2 (red) genes are indicated. (F) GO comparison of genes belonging to Cluster 1 and Cluster 2. Size of the circles represents the ratio of genes present in each GO category and color code the associated p-value representing the probability of seeing at least x number of genes out of the total n genes in the list annotated to that GO term. (G) A zoomed view of the transition profile heatmap restricted to genes from cluster 2 that belong to the “actin binding” GO category. Respective Tr1 and Tr2 fold changes are indicated on the heatmap. (H) In situ hybridisation showing that Gel and dCryAB that are part of the “actin binding” GO class are predominantly expressed in LT muscles. Lateral views of stage 15 embryos are shown. Anti-actin or anti-β3 tubulin antibodies are used to reveal muscle pattern. Schemes of muscles in an abdominal segment are shown with Gel and dCryAB expression indicated by a color code: red – high expression and light pink – low expression levels.

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