Figure 2 | Scientific Reports

Figure 2

From: Immunodominant T-cell epitopes from the SARS-CoV-2 spike antigen reveal robust pre-existing T-cell immunity in unexposed individuals

Figure 2

T-cell reactivity to SARS-CoV-2 Spike peptide pools and OncoPeptVAC prioritized peptides. Reactivity was determined by intracellular IFN-γ staining and surface expression of 4-1BB by FACS after stimulation of PBMCs from unexposed donors (n = 14) using separate pools of Spike-S1 and S2 peptides and the 11-Peptide-mix predicted by OncoPeptVAC (see “Methods”). (A) Structure of Spike—ACE2 receptor complex showing the location of the 11-peptides predicted by OncoPeptVAC. (B-C) T-cell activation after 48 h incubation with the peptides. (D-E) T-cell activation after a 7-day incubation with the peptides. (F) Kinetics and magnitude of CD8 T-cell activation in unexposed donor PBMCs. (G,H) CD8 T-cell response of donors D167 and D089 to individual peptides from the 11-peptide-mix. Statistical significance determined by Wilcoxon matched pairs signed rank test in Figures (B-E).

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