Figure 1
Expression and activity of ATF6β. (A,B) Expression of Atf6b mRNA in normal tissues (n = 5 mice) (A) and in cultured cells (n = 3–8) (B). HPC: hippocampus, Cx cerebral cortex, SC spinal cord. Total RNA was isolated from the indicated samples and qRT-PCR was performed. Data are shown as mean ± SEM. *p < 0.05, ***p < 0.001 by a one-way ANOVA followed by the Tukey test. (C) In situ hybridization (upper panel) and in situ hybridization-immunohistochemistry (lower panel) of Atf6b mRNA in the normal brain. Images in the right panels are enlarged views of the CA3 area. Scale bars: 200 μm (left panels) and 25 μm (right panels). Typical images from three independent experiments are shown. (D) qRT-PCR analysis of expression of Atf6b mRNA in primary hippocampal neurons under ER stress. Cells were treated with Tg (300 nM) or Tm (1 μg/ml) for 8 h and then qRT-PCR was performed. n = 3. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01 by a one-way ANOVA followed by the Tukey test. (E) Activation of ATF6β by ER stress in primary hippocampal neurons. Cells were treated with Tg (300 nM) for 2 h or DTT for 1 h. Extracted proteins were subjected to western blotting. The typical data from two independent experiments are shown. FL full length, NTF N-terminal fragment.
