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Figure 1

From: Influence of sub-inhibitory concentrations of antimicrobials on micrococcal nuclease and biofilm formation in Staphylococcus aureus

Figure 1

Biofilm characteristics of S. aureus ATCC12600, Newman lux, and Newman lux Δnuc1. (A) Biomass of biofilms measured by spectral absorption after crystal violet staining. (B) Polysaccharide content of biofilms measured by fluorescent intensity after calcofluor white staining (C) Colony forming units (CFU’s) per cm2 of biofilms grown for 24 h in a 24 wells plate. (D) Ratios of biomass/CFU and (E) polysaccharides/CFU of biofilms. All data shown are averages of triplicate measurements in three separate cultures, repeated on separate days. Error bars indicate standard error of the mean. Statistical differences were calculated using an ANOVA. *Significant difference (p < 0.05). In (D, E) the natural logarithm of the values was used for statistical analysis as the data are lognormally distributed (see also Fig. S3).

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