Figure 3

Change in biomass per CFU and polysaccharides per CFU ratios of S. aureus ATCC12600 and Newman lux biofilms. Biomass is quantified by crystal violet staining and subsequent measurement of spectral absorption at 590 nm. Polysaccharides are quantified by calcofluor white and subsequent measurement of fluorescent intensity at 355/490 nm (Ex/Em). Values shown is increase relative to the control biofilms without antibiotics (= 1; dotted horizontal line). Data shown are based on averages of triplicate measurements, repeated on separate days. Error bars indicate standard error of the mean. Statistical differences were calculated using an ANOVA comparing lognormal values (see “Materials and methods”). *Significant difference when compared to the culture without antibiotics (*p < 0.05; **p < 0.01; ***p < 0.001).