Figure 1
PBM treatment improves burn wound healing. (a) Representative diagram showing scheme of wound healing experiment. On the 1st day, the dorsal skin of 5-week-old C57BL/6NCr male mice was shaved, and on the 2nd day, two burn wounds were created, followed by PBM treatments (Day 0) with an 810 nm CW diode laser. Burn wounds were photographed every other day up to day 9; (b) Tissue surface temperature was monitored during laser treatments with increasing irradiances with a thermal camera; (c) Tissue damage was assessed in these tissues with TUNEL staining indicating phototoxicity at skin temperature above 45 °C; (d) Burn wound healing following PBM treatments at increasing doses and concomitant surface cooling, results are expressed as means and SDs representative of two independent experiments, significance was determined using non-parametric Student's t-test with *p-value < 0.05; (e) Optimal PBM dose treatments ensured skin surface temperature was > 45 °C using a dynamic irradiance protocol assessed with thermal imaging (left) and quantitation (right); (f) PBM treatments on burn wounds were photographed every other day for up to 9 days and compared to untreated controls; (g) Wound areas were digitally quantitated, results are expressed as means and SDs that is representative of five independent experiments, significance was determined using non-parametric Student's T-test with n = 8, *p-value < 0.05.
