Figure 3
PBM activated myofibroblasts in burn wounds promotes wound contraction. (a) PBM treated mice were sacrificed, and wound areas were immunostained for α-SMA on day 9; (b) Digital quantitation of immunohistochemical staining from mice sections, means and SDs are shown (n = 8, unpaired T-Test, **p < 0.005); (c) Human dermal fibroblast cells were plated in a 6-well tissue culture plate and were allowed to form confluent cultures for 24 h, and a scratch wound was created. PBM treatments at different doses with or without SB431542 inhibitor was performed, and images were captured with a digital microscope at 12 h; (d) Images were quantitated using T scratch software, and % area closed are shown as means and SDs that is representative of two independent experiments performed with replicates, significance was determined using one-way ANOVA among different treatments using the Tukey's multiple comparisons test indicated as *p < 0.05 and n.s. not significant; (e) Collagen gel contraction assays were performed with dermal fibroblast cells cast in collagen gels plated in 24-well culture dishes. PBM treatments were performed at various doses with or without prior incubation with SB431542, and gels were then photographed after 24 h; (f) Gel areas were plotted, and data is shown as means and SDs that is representative of two independent experiments performed with replicates, statistical significance was determined with one-way ANOVA among different treatments using the Tukey's multiple comparisons tests, **p < 0.005; (g) Gels were fixed and immunostained for αSMA, and representative fluorescence images are shown.
