Figure 4
PBM treatments increased macrophage phagocytic activity. (a) Burn wound tissues from untreated and PBM treated mice at 24 h post-injury were immunostained for the macrophage markers F4/80 and Mac-2; (b) Quantitation of F4/80 immunohistochemical staining from mice sections is represented as means and SDs (n = 8, unpaired Student's T-Test, n.s. not significant); (c) Quantitation of Mac-2 immunohistochemical staining is shown as means and SDs are shown (n = 8, unpaired Student's T-Test, n.s. not significant); (d) Macrophage cell line RAW 264.7 cells were seeded in 96 well plates and incubated with FITC-labelled latex beads to examine phagocytosis. Cell membranes were counterstained with Texas Red-conjugated Wheat Germ Agglutinin and imaged using a fluorescence microscope; (e). Fluorescent intensity was quantitated using NIH ImageJ, and data are presented as means and SDs that are representative of two independent experiments performed with replicates; statistical significance was determined with one-way ANOVA among different treatments using the Tukey's multiple comparisons test, *p < 0.05.
