Figure 5
PBM downregulates inflammatory response in burn wounds. (a) Laser treatments were performed at varying doses and 24 h later mice were anesthetized and injected with the inflammation detection probe (XenoLight RediJect, 200 mg/kg, 100 μl per mice, i.p.) and live fluorescence imaging was performed; (b) Quantitation of fluorescence images using the Living Image software (Perkin-Elmer) and results are shown as means and SDs representative of two independent experiments with replicates, significance was determined using non-parametric Student's t-test with *p value < 0.05; (c) qPCR arrays were performed on wound tissues at 24 h of post-PBM treatments using inflammasome array and data is shown as a pair-wise comparison of gene expression in control versus PBM treated mice burn wounds with XY-scatterplot analysis of log base 2-transformed expression data, results represent two independent studies performed with triplicates; each dot represents a gene, with red dots showing genes denoting downregulated genes following PBM treatments with an FDR corrected p < 0.05, green dots represents upregulated genes with FDR < 0.05 and black dots are genes whose expression was similar between the two groups; (d) Hierarchical clustering of differentially regulated genes in untreated versus PBM treated burn wounds in the inflammasome array; (e) Similar qPCR arrays were performed on wound tissues at 24 h of post-PBM treatments using the inflammatory response and autoimmunity array and data is similarly presented as described above; (f) Hierarchical clustering of differentially regulated genes in the latter gene expression array.
