Figure 6
Lack of PBM-activated TGF-β fails to promote burn wounds in chimeric mice. (a) Schematic for wild type latent TGF-βL1β1/L1β1 and chimeric latent TGF-βL1β3/L1β3 knock-in mouse models; (b) MEFs obtained from both mice were PBM treated, lysed, and assessed for phospho-Smad2 by western blotting, recombinant TGF-β1 treatment was used as a positive control; (c) Burn wound healing and PBM treatments were performed in the WT mice, and digital images were captured for up to 7 days; (d) Wound areas were digitally quantitated, results are expressed as means and SDs representative of two independent experiments, n = 6, significance was determined using non-parametric Student's t-test with *p-value < 0.05; (c) Similarly, burn wound healing and PBM treatments were performed in the KI mice, and digital images were captured for up to 7 days; (d) Wound areas were digitally quantitated, results are expressed as means and SDs representative of two independent experiments, n = 6, significance was determined using non-parametric Student's t-test with, n.s. not significant.
