Figure 2

PGE2 regulates the expression of surface receptors on PBMC and neutrophils from HD and TB patients. (A–E) PBMC from HD (n = 7) and TB patients (n = 11) were stimulated with Mtb-Ag (10 µg/ml) in the presence or absence of PGE2 (2 µM) for 5 days. Afterwards, the surface expression of (A) SLAMF1 and (B) CD31 on CD3⁺ T cells and (C) CD80, (D) MHC-I and (E) MHC-II on CD14⁺ mononuclear phagocytes were determined by flow cytometry. Each bar represents the mean percentage of (A–C) CD3⁺ or (D,E) CD14⁺ Receptor⁺ cells ± SEM or relative Mean Fluorescence Intensity (rMFI) ± SEM. (F–H) Human purified neutrophils from HD (n = 7) and TB patients (n = 6) were stimulated with Mtb-Ag (10 µg/ml) in the presence or absence of PGE2 (2 µM) for 2 h. Then, (F) SLAMF1, (G) PD-L1 and (H) PD-L2 expressions were evaluated by flow cytometry. Bars represent the mean values of the percentage of Receptor⁺ neutrophils ± SEM. Statistical differences were calculated using one-way ANOVA and post hoc Dunnett’s multiple comparison test. * p < 0.05. # p < 0.05; Mann–Whitney nonparametric test for unpaired samples.