Table 1 The effect of maximum January temperature (C), annual rainfall (mm), number of rainy days (> 0.1 mm/day), and length of hydroperiod (days) on genetic diversity measures (mean number of alleles/locus, Shannon's information evenness, and observed heterozygosity).

Diversity measure	Number of alleles/locus (Na)			Shannon's evenness (J)			Observed heterozygosity (Ho)
Diversity measure	Estimate	Wald \({\upchi }_{1}^{2}\)	P	Estimate	Wald \({\upchi }_{1}^{2}\)	P	Estimate	Wald \({\upchi }_{1}^{2}\)	P
Maximum January temperature (MJT)	0.07	0.04	0.848	− 0.28	10.29	0.001	− 0.060	9.53	0.002
Annual rainfall (AR)	− 0.40	7.82	0.005	0.11	1.27	0.259	0.010	0.13	0.719
Annual rain days (RD)	− 0.55	18.46	< 0.001	− 0.05	4.20	0.041	0.021	2.85	0.091
Hydroperiod (HP)	0.06	0.35	0.557	0.06	2.75	0.097	0.031	20.05	< 0.001
MJT * AR	− 0.83	7.21	0.007	0.19	1.90	0.168	0.056	2.96	0.085
MJT * RD	− 0.37	13.33	< 0.001	− 0.05	2.01	0.156	− 0.040	10.15	0.001
MJT * HP	0.05	0.02	0.890	− 0.03	0.41	0.523	− 0.078	7.84	0.005
AR * RD	− 0.29	10.93	0.001	0.08	0.73	0.394	0.096	9.05	0.003
AR * HP	− 0.01	0.03	0.858	0.09	0.57	0.451	0.098	6.10	0.014
RD * HP	0.50	4.50	0.034	0.24	5.13	0.024	0.066	4.16	0.041
Annual sample size	0.02	8.84	0.003	0.02	2.91	0.088	0.002	0.93	0.334

Mean number of alleles/locus and the four climatic variables were standardized within pool. Significant effects are indicated in bold.

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