Figure 3
From: Gene expression profiles during tissue remodeling following bladder outlet obstruction

Expression levels of Dbp in the three experimental groups. (A) Relative Dbp mRNA levels in the rat urinary bladder in the control, MH, and SH groups. mRNA levels were quantified by RT-pPCR. Measurements were normalized to Actb mRNA levels. Data are means ± SEM. *P < 0.05. (B) Immunohistochemistry using anti-DBP antibodies. DBP was stained brown via DAB immunolabeling (white arrowheads). Nuclei were stained blue by hematoxylin stain. All images are obtained using AdvanView 3.7 imaging software (http://advan-vision.com). Scale bars: 10 mm. (C) The number of DBP-positive cells in immunostaining images was quantified using ImageJ. Data are means ± SEM (n = 4). *P < 0.05.