Figure 2 | Scientific Reports

Figure 2

From: MCT4 is induced by metastasis-enhancing pathogenic mitochondrial NADH dehydrogenase gene mutations and can be a therapeutic target

Figure 2

Effect of MCT4 knockdown on the invasiveness of P29mtB82M cells. (a) Western blot analysis of the expression of MCT4 in P29mtB82M cells transfected with a control siRNA (siCont) or an MCT4 siRNA (siMCT4 #1 and #2). β-Actin was used as a loading control. Uncropped Western blot images are shown in Supplementary Fig. S12. (b) Proliferation of P29mtB82M cells transfected with a control siRNA (siCont) or an MCT4 siRNA (siMCT4 #1 and #2), as measured by WST assay. The cells were cultured for 2 days. Error bar: SD. (c) Gelatine invadopodia assay. Images showing the degradation of FITC-labelled gelatine at the invadopodia of P29mtB82M cells transfected with a control siRNA (siCont) or an MCT4 siRNA (siMCT4 #1 and #2). Bar. 20 μm. (d) Quantitation of the degraded area/cell in the invadopodia assay. Error bar: SD. (e) Matrigel invasion assay. Images showing invasion of P29mtB82M cells transfected with a control siRNA (siCont) or an MCT4 siRNA (siMCT4 #1 and #2). (f) Quantitation of the number of invaded cells. Error bar: SD. (g) RT-qPCR analysis of the expression of MCT4 and matrix degradation-related genes in P29mtB82M cells transfected with a control siRNA (siCont) or MCT4 siRNAs (siMCT4 #1 and #2). Error bar: SD.

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