Figure 3

Effect of prior RV infection on clinical symptoms of acute DSS-induced colitis. (A). Schematic representation of the study timeline. Wild type C57BL/6NRj mice were categorized into 3 groups (control, only DSS and DSS with prior RV infected groups). Administered 1% to 3% DSS in drinking water for 7 to 9 days and followed by 1 day of normal drinking water. Mice were monitored daily. (B) Body weight was measured daily and means ± SD of percent body weight were plotted. (C) Colon length was measured as an indirect marker of inflammation. (D) Disease activity index was calculated as follows: DAI = (weight loss + stool consistency + gross bleeding)/3). Results are shown as mean ± SD. A total of 80 mice included from four independent experiments. All 2%, 2.5% and 3% DSS where administered for 7 days followed by 1 day with water (DSS started 6 days post RV infection). 1% DSS was administered for 8 days followed by 1 day with water (DSS started 14 days post RV infection) and 1.5% DSS was administered for 9 days followed by 1 day with water (DSS started 6 days post RV infection).