Figure 4

Effects of DSS-induced colitis on the recruitment of monocytes to the colon. Monocyte infiltration into the colon was assessed after induction of inflammation by DSS. (A) Representative gating scheme from 1% DSS (RV-14 days experiment, 9 days post DSS onset). Representative expression of CD11b and Ly6G from live-gated CD45⁺ colonic cells (upper panels), expression of CD64 and Ly6C from CD11b⁺Ly6G^- colonic cells (middle panels), and expression of MHCII and Ly6C from CD11b⁺Ly6G^-CD64⁺Ly6C^hi/lo (lower panels) in control, only DSS and DSS with prior RV infected groups. Neutrophils are shown as CD11b⁺Ly6G^hi. Monocytes are defined as CD11b⁺Ly6G^-CD64⁺Ly6C^hiMHCII^+/-. Macrophages are defined as CD11b⁺Ly6G^-CD64⁺Ly6C^loMHCII⁺. (B) Frequency and absolute numbers of neutrophils in the control, DSS or DSS + RV groups. (C) Frequency and absolute numbers of monocytes in the control, DSS or DSS + RV groups. (D) Frequency and absolute numbers of macrophages in the control, DSS or DSS + RV groups. (E) Ratios of Monocyte: Macrophage in the control, DSS or DSS + RV groups. Results are shown as mean ± SD and are derived from two independent experiments with 4–5 mice per group. All data points indicate individual mice. Black symbols indicate data from the experiment in which 1% DSS was administered for 8 days followed by 1 day of water (DSS started 14 days post RV infection), grey symbols indicate data from the experiment in which 1.5% DSS was administered for 9 days followed by 1 day with water (DSS started 6 days post RV infection). (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).