Figure 5
From: Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
Impact of the orientation and nucleotide composition of connecting loops on the differential enrichment of proteins on G4 structures. Western-blotting analysis and quantification of the interaction of proteins found enriched on constrained G4 structures with modified molecules (1a, 1b, 2, 3, 4) and with unconstrained G4 (c-myc and 21 T). Arrows indicate the 5′-3′ strand orientation of single-stranded extensions or connecting loops present on different systems. The modification of the nucleotide composition of connecting loops in the system 4 is indicated by the sequence TCT. Construct 8 and scramble sequence were used as control for pull-down performed with constrained of free-G4 structures, respectively.
