Figure 2

Isolation and characterization of human placental cells. (a,b) Microscopic images of human placental cells at low (a) and high (b) confluency. (c) Normal 46,XX karyotype of placental cells at passage 11. (d–f) Representative flow cytometry analysis, showing high expression level of EPC markers compared to unstained cells. (g) Placental cell migration in response to VEGF. Histogram is representative of cell counts in four random microscopic fields from two independent experiments (at passages 7 and 13). Bars indicate means ± SD (P < 0.01, non-parametric signed-rank test). Scale bars, 200 µm (a,b).