Figure 3 | Scientific Reports

Figure 3

From: Generation of vascular chimerism within donor organs

Figure 3

Cell seeding and generation of vascular chimerism in vitro. (a) H&E staining of human placental EPCs seeded on completely decellularized human umbilical vein. (b,c) H&E (B) and corresponding vWF immunohistochemistry staining (c) of human placental EPCs on de-endothelialized rat aorta. (d-f) SEM images of untreated (d), de-endothelialized and human EPC-seeded (f) rat aorta. (g) Corresponding high magnification SEM image of EPC-seeded aorta. Yellow arrows show cell-to-cell connections, yellow arrowheads show cell-to-ECM connections. Scale bars, 100 µm (a,c) and 50 µm (b).

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