Figure 3
Expression of STAT5a phospho-mutants differentially affects PRL-regulated gene expression in MCF7 cells. (A) Supervised clustering of each STAT5a species’ top 50 DEGs (≥ 1.5-fold change) from RNA-seq analysis of PRL-treated versus untreated MCF7 cells expressing STAT5a rescues using the heatmap2 tool on www.usegalaxy.org (Galaxy Version 3.0.1). (B) IPA functional pathway enrichment analysis shows upstream regulators and predicted pathway activation or inhibition with PRL treatment of WT-STAT5a-expressing MCF7 cells. (C–E) IPA functional pathway analysis of indicated STAT5a phospho-mutants overlaid with the regulatory network shown in panel B to predict activation or inhibition. p value calculated by Fisher’s exact test for networks generated in IPA with predicted effects based on the similarity of gene expression values to annotated network relationships in the Ingenuity knowledge base (IPA software 2020 Version 62,089,861, Qiagen).
