Figure 3
From: Concurrent YAP/TAZ and SMAD signaling mediate vocal fold fibrosis
Verteporfin inhibited TGF-β1-induced YAP/TAZ activation in HVOX fibroblasts. HVOX fibroblasts were treated with or without 10 ng/mL TGF-β1 and 0–100 µM verteporfin (VP) for 24 h. Expression of ACTA2 (a), target genes of YAP/TAZ (b CCN2; c CYR61: d EDN1; e FSTL1; f INHBA), YAP1 (g), and WWTR1 (h) were analyzed by qPCR. Cytotoxicity of VP was analyzed by LDH (i) and LIVE/DEAD cell viability assays (j; data shown as mean ± SD in i and j; n = 5; *p < 0.05, Dunnett's test). Representative images of live (green) and dead (red) cells respectively stained with calcein AM and ethidium homodimer-1 (k; bar = 100 µm).
