Figure 4
From: Concurrent YAP/TAZ and SMAD signaling mediate vocal fold fibrosis
Verteporfin inhibited TGF-β1-induced fibroplastic phenotype changes in HVOX fibroblasts and rat VFFbs. HVOX fibroblasts and rat VFFbs were treated with or without 10 ng/mL TGF-β1 and 3 µM verteporfin (VP) for 24 h. Expression of CCN2 (a), EDN1 (b), FSTL1 (c), ACTA2 (d), COL1A1 (e), FN1 (f), and SERPINE1 (g) in HVOX and Ccn2 (h), Edn1 (i), Fstl1 (j), Acta2 (k), Col1a1 (l), Fn1 (m), and Serpine1 (n) in rat VFFbs were analyzed by qPCR (data shown as mean ± SD; n = 3; *p < 0.05, control vs VP, TGF-β1 vs TGF-β1 + VP, Student’s t-test). Expression of αSMA (red) and collagen proteins (green) was evaluated by immunostaining (o; bar = 100 µm) and Western blotting (p; representative grouping of gels cropped from different parts of the same gel or from different gels; whole gels available in supplemental material). Cell nuclei counterstained with DAPI (blue; o).
