Figure 8
From: Concurrent YAP/TAZ and SMAD signaling mediate vocal fold fibrosis
Verteporfin inhibited TGF-β1-induced SMAD2/3 nuclear accumulation without interfering with phosphorylation of SMAD2/3. HVOX fibroblasts were treated with or without 10 ng/mL TGF-β1 and 3 µM verteporfin (VP) for 24 h. Western blotting was performed to detect Ser465/467-phosphorylated SMAD2 (pSMAD2), SMAD2, Ser423/425-phosphorylated SMAD3 (pSMAD3), SMAD3, and β-actin in the total cell fraction (a; representative grouping of gels cropped from different parts of the same gel or from different gels; whole gels available in supplemental material). Nuclear localization of YAP/TAZ and SMAD2/3 were examined by immunostaining (b; bar = 100 µm).
