Figure 3
Gavage administration of GLAA led to alterations in gut metabolites that were correlated with sleep behaviour in mice. (a) PCA score plots of metabolome profiles of the control group and the 100 mg/kg GLAA group. (b) OPLS-DA score plots of metabolome profiles of the control group and the 100 mg/kg GLAA group. (c) Correlations of the top 30 VIP (variable importance in projection) gut metabolites, sleep behaviour and the concentration of 5-HT in the hypothalamus were determined using Spearman’s rank correlation analysis. The image is generated by SIMCA 14.1 and R package “corrplot”. The results with p values < 0.05 are shown in the heat map. The colour key and circle size indicate the strength of the correlation (r value). Red indicates positive correlations; blue indicates negative correlations. The data are calculated from 8 samples per group. Except specifically mentioned, the images are generated by SIMCA 14.1. GLAA the acidic part of the alcohol extract of G. lucidum mycelia, PCA principal component analysis, OPLS-DA orthogonal partial least squares discriminant analysis, VIP variable importance in projection, 5-HT 5-hydroxytryptamine.
