Figure 2

MAVS is required for the type I and III IFN response to SARS-CoV-2. (A,B) Calu-3 cells were depleted of MAVS by lentiviral shRNA delivery using two independent shRNAs (shRNA-MAVS-06 and shRNA-MAVS-45). Knockdown efficiency was assessed by western blot (A) and RT-qPCR (B). The control shRNA targeted GFP, which is absent in Calu-3 cells. See Supplementary Figure 3 for full blots. (C,D) Calu-3 cells depleted of MAVS using shRNA-MAVS-45 were infected with SARS-CoV-2 (MOI = 0.1) for 48 h, followed by RNA extraction and RT-qPCR for the indicated transcripts. Data are relative to GAPDH expression. Data in (A,B) are representative of two independent biological repeats. Data in (C,D) are pooled from 3 independent biological repeats, with bars representing the average. Data were analysed by t-test and significant differences are indicated (**P < 0.01; *P < 0.05).