Figure2
Phenotypic resilience in pooled CRISPR/Cas9 library infected iPS cells. Uninfected iPS cells (control) or those transduced with CRISPR/Cas9 library (virus) were assessed for (a) gene expression of pluripotency markers (n = 3 per condition), (b) proliferative potential (n = 3 per condition), and (c) cardiac troponin T (cTNT) protein expression after differentiation to cardiomyocytes. (d) Percent of CRISPR/Cas9 library sgRNAs present in iPS cells at 10 read-counts or higher. (e) Number of sgRNAs per gene present in plasmid, iPS cells, and iPS derived cardiomyocytes.
