Figure 4
From: Relevance of circulating hybrid cells as a non-invasive biomarker for myriad solid tumors
Multiplexed phenotypic analysis of tissue biopsy and CHCs from a subject with relapsed refractory breast cancer. (A) Cyclic immunofluorescence microscopy evaluation of tumor biopsy tissue before and during treatment reveals hot spots of viable disease. (B) Unsupervised clustering [k-means clustering with the number of clusters (n = 20)] of cellular immunophenotype identified distinct clusters and reveal tumor heterogeneity. (C) Sankey diagram of tumor phenotypes [proliferative (Ki67), stem (CD44), hormonal status (AR, HR)] quantifies heterogeneity of epithelial marker positive tumor cells. (D) Representative images of heterogenous populations of hybrid cells expressing tumor epithelial (CK and/or ECAD) and immune (CD45) markers within biopsied tumor and (E) peripheral blood mononuclear cells. Heatmap created with MathWorks MATLAB ver. R2017b, www.mathworks.com.
