Figure 3
Taxol attenuated VI-17-elicited cytotoxicity on HCC cells. (A) Flow cytometric analysis for the effect of VI-17 on cell cycle. (B) Flow cytometric analysis for the effect of VI-17 on cell apoptosis. (C) Western blot analysis for expression of apoptotic molecules. (D) IC50 values of VI-17 on hepatoma cells at indicating time points according to MTT assays. Data are expressed as Mean ± SD of three independent experiments in triplicates. *p < 0.05. (E) MTT assays for the effects of z-VAD-fmk, necrostatin-1, taxol and low temperatures on VI-17 induced cell death of Hep3B cells. (F) Analysis for the ratio of PI-positive cell under PEGs pretreatment in VI-17-treated Hep3B cells. Data are expressed as Mean ± SD of three independent experiments in triplicates. *p < 0.05.
