Figure 7
VI-17 interacted with tubulin-α, and induced tubulin-α degradation and porimin exposure. (A) Western blot analysis of candidate binding partners of VI-17 in pull-down mixture using biotin-VI-17 from Hep3B cells. (B) Immunofluorescence for cellular location of VI-17 and tubulin-α in VI-17 treated Hela cells. Original magnification: × 400. (C) Immunofluorescence for tubulin-α degradation and mitochondria swell in VI-17 treated Hep3B cells. Original magnification: × 200. (D) Western blot analysis of the expression of tubulin-α and porimin in VI-17 treated Hep3B cells at indicating time points. (E) Gray value analysis for the band in D. *p < 0.05.
