Figure 6
In silico analyses of AtCOI1-JA-Ile-AtJAZ1 (PDB ID: 3OGL, a,d,g), SlCOI1-JA-Ile-SlJAZ1 (b,e,h), and SlCOI1-JA-Ile-SlJAZ5 (c,f,i) to show the binding mode of the extended degron. (a–c) The amino acid residues forming hydrogen bonds (H-bond) or hydrophobic interaction (Hydrophobic) between COI1 and JAZ observed in the crystal structure of AtCOI1-JA-Ile-AtJAZ1 (3OGL, a), MD simulation of SlCOI1-JA-Ile-SlJAZ1 (b) and MD simulation of SlCOI1-JA-Ile-SlJAZ5 (c). (d) The reported structure around the degron sequence of AtJAZ1 in the complex of AtCOI1-JA-Ile-AtJAZ1. (e,f) The obtained MD structure around the degron sequence of SlJAZ in the complex of SlCOI1-JA-Ile-SlJAZ1 (e) or SlCOI1-JA-Ile-SlJAZ5 (f). (g) The reported structure around the DOD sequence of AtJAZ1 in the complex of AtCOI1-JA-Ile-AtJAZ1. (h,i) The obtained MD structure around the DOD sequence of SlJAZ in the complex of SlCOI1-JA-Ile-SlJAZ1 (h) or SlCOI1-JA-Ile-SlJAZ5 (i). Hydrogen bonds are shown as yellow dotted lines. The images of the 3D structures were created by MOE 2020.09.
