Figure 2
(A) Freezing curves of the l-lactate dehydrogenase (LDH) solution (20 μg/mL, 0.2 M Tris–HCl buffer, pH 7.4) at different liquid-mediated freezing (LMF) conditions [Ti] = − 10, − 20, − 30, − 40, or − 50 °C). Ti, the temperature of isopropyl alcohol just before freezing LDH samples. (B) Effect of freezing rate on the activity of LDH dissolved in 0.2 M Tris–HCl buffer (pH 7.4). Results are expressed as the means ± SDs. Asterisks (*) indicate significant differences (p < 0.05). NS non-significant difference.
